[ 290ANCHLIAP19 ] PR Lab Course in Instrumental Analysis

Es ist eine neuere Version 2023W dieser LV im Curriculum Bachelor's programme Chemistry and Chemical Technology 2023W vorhanden.
Workload Education level Study areas Responsible person Hours per week Coordinating university
5 ECTS B3 - Bachelor's programme 3. year Chemistry Wolfgang Buchberger 5 hpw Johannes Kepler University Linz
Detailed information
Pre-requisites PR Lab Course in Analytical Chemistry VL Instrumental Analytical Chemistry
Original study plan Bachelor's programme Chemistry 2018W
Objectives Practical experience in using modern instruments for analytical chemistry
Subject Students will get practical experience with state-of-the-art analytical instrumentation with a focus on chromatographic and electrophoretic techniques. Students have to work independently and carry out the following analyses:

  1. Determination of residues of pesticides in honey by gas chromatography / mass spectrometry (GC-MS). This analysis deals with organic trace analysis and includes sample preparation and analyte enrichment by liquid-liquid extraction. Gas chromatographic separation is hyphenated with mass spectrometric detection in the selected ion monitoring mode.
  2. Ion chromatographic determination of anions in drinking water. This analysis demonstrates the routine quantitation of inorganic anions like chloride, nitrate and sulfate by anion-exchange chromatography and suppressed conductivity detection.
  3. Quantitation of ingredients of commercially available pharmaceutical formulations by reversed-phase high-performance liquid chromatography (RP-HPLC) and UV detection. This analysis demonstrates quality control of pharmaceutical products.
  4. Determination of artificial sweeteners by capillary electrophoresis (CE). This analysis shows existing alternatives to widely used chromatographic techniques.
Criteria for evaluation Quality of practical work in the lab; Lab reports on practical work; Final oral exam
Methods Practical work in the chemical laboratory
Language English
Study material
  • J.W. Robinsons, E.M.Skelly Frame, G.M. Frame :Undergraduate instrumental analysis (7th ed.), CRC Press, 2014, ISBN 978-1-4200-6135-2
  • S. Pedrozzi, Practical Instrumental Analysis, Wiley, 2013, ISBN 978-3-527-32951-9
  • D.C.Harris : Quantitative Chemical Analysis (9th ed.), Freeman, 2016. ISBN 978-1-4641-3538-5
Changing subject? No
Corresponding lecture TCBPBPRANC2: PR Praktikum aus Analytischer Chemie II (6 ECTGS)
On-site course
Maximum number of participants 10
Assignment procedure Direct assignment