Inhalt
[ 600MTAROPFP25 ] PR Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins
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| Workload |
Education level |
Study areas |
Responsible person |
Hours per week |
Coordinating university |
| 3 ECTS |
R - Doctoral programme |
(*)Biophysik |
Peter Pohl |
2 hpw |
Johannes Kepler University Linz |
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| Detailed information |
| Pre-requisites |
(*)Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
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| Original study plan |
Doctoral programme Natural Sciences 2025W |
| Learning Outcomes |
Competences |
| Students learn standard molecular biology techniques for (membrane) protein purification and reconstitution.
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| Skills |
Knowledge |
| Students acquire practical skills in the overexpression, purification, and reconstitution of membrane proteins into lipid vesicles. They gain hands-on experience with key laboratory techniques such as affinity chromatography, detergent-mediated protein solubilization, and vesicle reconstitution. Additionally, they develop competencies in troubleshooting purification and reconstitution protocols, optimizing experimental conditions, and analyzing protein integrity and activity using biochemical and biophysical assays.
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Protein overexpression, purification, and reconstitution into lipid vesicles.
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| Criteria for evaluation |
The final grade will be determined by a combination of the students' preparation and execution of the experiment and the quality of the written protocols.
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| Methods |
Experimental techniques in molecular biology.
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| Language |
English |
| Changing subject? |
No |
| Further information |
The course is part of a comprehensive experimental cycle in membrane transport protein research, covering key stages of investigation. This cycle includes protein production and reconstitution into vesicles (“Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins”), assessment of reconstitution efficiency (“Fluorescence Correlation Spectroscopy”), and functional characterization of the protein (“Single Channel Recordings”). Participation in the other two thematic courses is recommended but not mandatory.
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| On-site course |
| Maximum number of participants |
5 |
| Assignment procedure |
Direct assignment |
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