Inhalt
[ 600MTAROPFP25 ] PR Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins
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| (*) Leider ist diese Information in Deutsch nicht verfügbar. |
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| Workload |
Ausbildungslevel |
Studienfachbereich |
VerantwortlicheR |
Semesterstunden |
Anbietende Uni |
| 3 ECTS |
R - Doktorat / PhD |
Biophysik |
Peter Pohl |
2 SSt |
Johannes Kepler Universität Linz |
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| Detailinformationen |
| Anmeldevoraussetzungen |
Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
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| Quellcurriculum |
Doktoratsstudium Naturwissenschaften 2025W |
| Lernergebnisse |
Kompetenzen |
| (*)Students learn standard molecular biology techniques for (membrane) protein purification and reconstitution.
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| Fertigkeiten |
Kenntnisse |
| (*)Students acquire practical skills in the overexpression, purification, and reconstitution of membrane proteins into lipid vesicles. They gain hands-on experience with key laboratory techniques such as affinity chromatography, detergent-mediated protein solubilization, and vesicle reconstitution. Additionally, they develop competencies in troubleshooting purification and reconstitution protocols, optimizing experimental conditions, and analyzing protein integrity and activity using biochemical and biophysical assays.
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(*)Protein overexpression, purification, and reconstitution into lipid vesicles.
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| Beurteilungskriterien |
(*)The final grade will be determined by a combination of the students' preparation and execution of the experiment and the quality of the written protocols.
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| Lehrmethoden |
(*)Experimental techniques in molecular biology.
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| Abhaltungssprache |
Englisch |
| Lehrinhalte wechselnd? |
Nein |
| Sonstige Informationen |
(*)The course is part of a comprehensive experimental cycle in membrane transport protein research, covering key stages of investigation. This cycle includes protein production and reconstitution into vesicles (“Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins”), assessment of reconstitution efficiency (“Fluorescence Correlation Spectroscopy”), and functional characterization of the protein (“Single Channel Recordings”). Participation in the other two thematic courses is recommended but not mandatory.
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| Präsenzlehrveranstaltung |
| Teilungsziffer |
5 |
| Zuteilungsverfahren |
Direktzuteilung |
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