| Detailed information |
| Pre-requisites |
(*)Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
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| Original study plan |
Doctoral programme Natural Sciences 2025W |
| Learning Outcomes |
Competences |
| Upon successful completion of the PR, students will be familiar with the c experimental technique and instrumentation of Fluorescence correlation spectroscopy. They will be able to design and carry out experiments aiming to detect the concentration and diffusion time of fluorescently labeled macromolecules, analyze and critically evaluate results.
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| Skills |
Knowledge |
- Calibration of the confocal volume with fluorescent standards
- Design and conduction of a typical FCS measurement of the labeled biopolymer
- Data processing using physical diffusion models
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Characterization of fluorescently labeled macromolecules, specifically, their diffusion coefficient, concentration and oligomeric state;
Obtaining the reconstitution efficiency of fluorescently labeled membrane proteins.
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| Criteria for evaluation |
The final grade will be determined by a combination of the students' preparation and execution of the experiment and the quality of the written protocols.
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| Methods |
Individual preparation check by the supervisor in the form of discussion; Execution of the experiments under supervision; processing the data and writing the protocol; discussion of the results with the supervisor.
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| Language |
English |
| Changing subject? |
No |
| Further information |
The course is part of a comprehensive experimental cycle in membrane transport protein research, covering key stages of investigation. This cycle includes protein production and reconstitution into vesicles (“Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins”), assessment of reconstitution efficiency (“Fluorescence Correlation Spectroscopy”), and functional characterization of the protein (“Single Channel Recordings”). Participation in the other two thematic courses is recommended but not mandatory.
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