Inhalt
[ 663BICHBL112 ] Module Biochemistry Laboratory 1
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| Workload |
Mode of examination |
Education level |
Study areas |
Responsible person |
Coordinating university |
| 3 ECTS |
Accumulative module examination |
B2 - Bachelor's programme 2. year |
(*)Biologische Chemie |
Hana Tykalova |
USB Budweis |
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| Detailed information |
| Pre-requisites |
PR Lab Course in General Chemistry
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| Original study plan |
Bachelor's programme Biological Chemistry 2025W |
| Learning Outcomes |
Competences |
| The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
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| Skills |
Knowledge |
| Students know and understand the principles of the basic biochemical methods (k1, k2), can perform such experiments (k3), analyze the results (k4), and draw conclusions (k5). Students can apply this knowledge and design experiments for needed analyses (k6).
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Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification.
Chromatographic methods in Biochemistry - separation of proteins using one of the selected chromatographic methods, such as gel chromatography, ionex chromatography, or affinity chromatography.
Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes.
Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction.
SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Further information |
Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification.
Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography.
DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA.
Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes.
Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction.
SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Corresponding lecture |
ANTBIOCBC1U10: PR Biochemisches Praktikum 1 (3 ECTS)
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| Subordinated subjects, modules and lectures |
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