Inhalt
[ 663BICHBL112 ] Modul (*)Biochemistry Laboratory 1
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| (*) Leider ist diese Information in Deutsch nicht verfügbar. |
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| Workload |
Form der Prüfung |
Ausbildungslevel |
Studienfachbereich |
VerantwortlicheR |
Anbietende Uni |
| 3 ECTS |
Kumulative Modulprüfung |
B2 - Bachelor 2. Jahr |
Biologische Chemie |
Hana Tykalova |
USB Budweis |
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| Detailinformationen |
| Anmeldevoraussetzungen |
(*)PR Lab Course in General Chemistry
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| Quellcurriculum |
Bachelorstudium Biological Chemistry 2025W |
| Lernergebnisse |
Kompetenzen |
| (*)The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
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| Fertigkeiten |
Kenntnisse |
| (*)Students know and understand the principles of the basic biochemical methods (k1, k2), can perform such experiments (k3), analyze the results (k4), and draw conclusions (k5). Students can apply this knowledge and design experiments for needed analyses (k6).
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(*)Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification.
Chromatographic methods in Biochemistry - separation of proteins using one of the selected chromatographic methods, such as gel chromatography, ionex chromatography, or affinity chromatography.
Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes.
Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction.
SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Sonstige Informationen |
(*)Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification.
Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography.
DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA.
Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes.
Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction.
SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Äquivalenzen |
(*)ANTBIOCBC1U10: PR Biochemisches Praktikum 1 (3 ECTS)
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| Untergeordnete Studienfächer, Module und Lehrveranstaltungen |
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