(*) Unfortunately this information is not available in english.
Workload
Education level
Study areas
Responsible person
Hours per week
Coordinating university
2 ECTS
R - Doctoral programme
Human Medicine
Yong Yu
2 hpw
Johannes Kepler University Linz
Detailed information
Pre-requisites
(*)Zulassung zum Doktoratsstudium Medical Sciences
Original study plan
Doctoral programme Medical Sciences 2025W
Learning Outcomes
Competences
Students understand the epigenetic mechanisms underlying X chromosome inactivation and Barr body formation. They can apply immunofluorescence (IF) and RNA-FISH techniques to detect key molecular markers and analyze the results.
Skills
Knowledge
Identifying and explaining epigenetic regulatory mechanisms involved in X chromosome inactivation.
Performing immunofluorescence (IF) staining to detect histone modifications.
Conducting RNA-FISH staining to visualize long non-coding RNA (lncRNA) XIST.
Preparing slides for fluorescence microscopy and analyzing results.
Applying experimental techniques in molecular biology to study gene regulation.
Epigenetic regulation of X chromosome inactivation.
The role of long non-coding RNA XIST in gene silencing.
Mechanisms of histone modification in chromatin regulation.
Principles and applications of immunofluorescence (IF) staining.
RNA fluorescence in situ hybridization (RNA-FISH).
Criteria for evaluation
A minimum 75% attendance is required. Students need to write a scientific report describing brief experiment procedures and results (1-2 pages). The positive assessment is "participated successfully", the negative assessment is "participated without success".
Methods
Introduction and supervision of experimental skills in medical teaching laboratory
