Inhalt

[ 405ERSLEFBP22 ] PR Seeing the eXISTing epigenetic factors on a Barr body

Versionsauswahl
(*) Leider ist diese Information in Deutsch nicht verfügbar.
Workload Ausbildungslevel Studienfachbereich VerantwortlicheR Semesterstunden Anbietende Uni
2 ECTS R - Doktorat / PhD Humanmedizin Yong Yu 2 SSt Johannes Kepler Universität Linz
Detailinformationen
Anmeldevoraussetzungen Zulassung zum Doktoratsstudium Medical Sciences
Quellcurriculum Doktoratsstudium Medical Sciences 2024W
Ziele (*)Students will learn the epigenetic regulatory mechanisms in X chromosome inactivation and formation of a Barr body. Students will perform immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) stainings to detect histone modifications and long-noncoding RNA (lncRNA) XIST in human diploid fibroblast cells (cell culture practice is optional). Students will prepare slides and observe the staining results using fluorescent microscope.
Lehrinhalte (*)Knowledge on Barr body epigenetic regulation; long non-coding RNA XIST; IF staining; RNA-FISH staining; fluorescence microscope imaging
Beurteilungskriterien (*)A minimum 75% attendance is required. Students need to write a scientific report describing brief experiment procedures and results (1-2 pages). The positive assessment is "participated successfully", the negative assessment is "participated without success".
Lehrmethoden (*)Introduction and supervision of experimental skills in medical teaching laboratory
Abhaltungssprache Englisch
Lehrinhalte wechselnd? Nein
Präsenzlehrveranstaltung
Teilungsziffer 10
Zuteilungsverfahren Zuteilung nach Vorrangzahl