Inhalt

[ 405ERSLEFBP22 ] PR Seeing the eXISTing epigenetic factors on a Barr body

Versionsauswahl
(*) Unfortunately this information is not available in english.
Workload Education level Study areas Responsible person Hours per week Coordinating university
2 ECTS R - Doctoral programme Human Medicine Yong Yu 2 hpw Johannes Kepler University Linz
Detailed information
Pre-requisites (*)Zulassung zum Doktoratsstudium Medical Sciences
Original study plan Doctoral programme Medical Sciences 2024W
Objectives Students will learn the epigenetic regulatory mechanisms in X chromosome inactivation and formation of a Barr body. Students will perform immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) stainings to detect histone modifications and long-noncoding RNA (lncRNA) XIST in human diploid fibroblast cells (cell culture practice is optional). Students will prepare slides and observe the staining results using fluorescent microscope.
Subject Knowledge on Barr body epigenetic regulation; long non-coding RNA XIST; IF staining; RNA-FISH staining; fluorescence microscope imaging
Criteria for evaluation A minimum 75% attendance is required. Students need to write a scientific report describing brief experiment procedures and results (1-2 pages). The positive assessment is "participated successfully", the negative assessment is "participated without success".
Methods Introduction and supervision of experimental skills in medical teaching laboratory
Language English
Changing subject? No
On-site course
Maximum number of participants 10
Assignment procedure Assignment according to priority