(*) Leider ist diese Information in Deutsch nicht verfügbar.
Workload
Ausbildungslevel
Studienfachbereich
VerantwortlicheR
Semesterstunden
Anbietende Uni
2 ECTS
R - Doktorat / PhD
Humanmedizin
Yong Yu
2 SSt
Johannes Kepler Universität Linz
Detailinformationen
Quellcurriculum
Doktoratsstudium Doctoral Program in Medical Sciences 2023W
Ziele
(*)Students will learn the epigenetic regulatory mechanisms in X chromosome inactivation and formation of a Barr body. Students will perform immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) stainings to detect histone modifications and long-noncoding RNA (lncRNA) XIST in human diploid fibroblast cells (cell culture practice is optional). Students will prepare slides and observe the staining results using fluorescent microscope.
Lehrinhalte
(*)Knowledge on Barr body epigenetic regulation; long non-coding RNA XIST; IF staining; RNA-FISH staining; fluorescence microscope imaging
Beurteilungskriterien
(*)A minimum 75% attendance is required. Students need to write a scientific report describing brief experiment procedures and results (1-2 pages). The positive assessment is "participated successfully", the negative assessment is "participated without success".
Lehrmethoden
(*)Introduction and supervision of experimental skills in medical teaching laboratory
