| Detailed information |
| Original study plan |
Doctoral programme Doctoral Program in Medical Sciences 2023W |
| Objectives |
Students will learn the epigenetic regulatory mechanisms in X chromosome inactivation and formation of a Barr body. Students will perform immunofluorescence (IF) and RNA fluorescence in situ hybridization (RNA-FISH) stainings to detect histone modifications and long-noncoding RNA (lncRNA) XIST in human diploid fibroblast cells (cell culture practice is optional). Students will prepare slides and observe the staining results using fluorescent microscope.
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| Subject |
Knowledge on Barr body epigenetic regulation; long non-coding RNA XIST; IF staining; RNA-FISH staining; fluorescence microscope imaging
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| Criteria for evaluation |
A minimum 75% attendance is required. Students need to write a scientific report describing brief experiment procedures and results (1-2 pages). The positive assessment is "participated successfully", the negative assessment is "participated without success".
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| Methods |
Introduction and supervision of experimental skills in medical teaching laboratory
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| Language |
English |
| Changing subject? |
No |
