Studienhandbuch | PR Molecular biology I

Inhalt

Workload	Education level	Study areas	Responsible person	Hours per week	Coordinating university
3 ECTS	M2 - Master's programme 2. year	^(*)Biophysik	Christine Siligan	2 hpw	Johannes Kepler University Linz

Detailed information
Original study plan	Master's programme Biophysics 2021W
Objectives	students should get an insight into molecularbiology and common techniques in the lab dealing with DNA cloning and PCR. basics of DNA cloning structure of DNA plasmids PCR techniques in medical diagnostics and forensics working with biological material and laboratory equipment working under sterile conditions
Subject	The course is devided into 3 parts: 1. in-frame cloning of a fusion gene via restriction enzymes reading of plasmid maps restriction enzymes DNA gel electrophoresis DNA isolation and ligation transformation of E.coli cells DNA preparation 2. POC - point of care analysis sample preparation PCR analysis 3. STR analysis sample preparation amplification of STR D1S80 via PCR DNA gel electrophoresis analysis of allelic frequency
Criteria for evaluation	writing of protocols for the experiments including analysis of obtained results motivation during practical course
Methods	cloning using restriction enzymes Agarose gel electrophoresis DNA extraction Transformation of E.coli cells clonale expansion of E.coli cells DNA isolation PCR analytical methods
Language	^(*)Deutsch or English
Study material	Hand-outs of technical background protocols for experiments
Changing subject?	No

On-site course
Maximum number of participants	15
Assignment procedure	Assignment according to priority