Inhalt

[ TPMWBPRMOB1 ] PR Molecular biology I

Versionsauswahl
Workload Education level Study areas Responsible person Hours per week Coordinating university
3 ECTS M2 - Master's programme 2. year Physics Christine Siligan 2 hpw Johannes Kepler University Linz
Detailed information
Original study plan Master's programme Biophysics 2021W
Objectives students should get an insight into molecularbiology and common techniques in the lab dealing with DNA cloning and PCR.

  • basics of DNA cloning
  • structure of DNA plasmids
  • PCR techniques in medical diagnostics and forensics
  • working with biological material and laboratory equipment
  • working under sterile conditions
Subject The course is devided into 3 parts:

1. in-frame cloning of a fusion gene via restriction enzymes

              reading of plasmid maps
              restriction enzymes
              DNA gel electrophoresis
              DNA isolation and ligation
              transformation of E.coli cells
              DNA preparation

2. POC - point of care analysis

              sample preparation
              PCR
              analysis

3. STR analysis

              sample preparation
              amplification of STR D1S80 via PCR
              DNA gel electrophoresis
              analysis of allelic frequency
Criteria for evaluation writing of protocols for the experiments including analysis of obtained results motivation during practical course
Methods
  • cloning using restriction enzymes
  • Agarose gel electrophoresis
  • DNA extraction
  • Transformation of E.coli cells
  • clonale expansion of E.coli cells
  • DNA isolation
  • PCR
  • analytical methods
Language (*)Deutsch or English
Study material Hand-outs of technical background protocols for experiments
Changing subject? No
On-site course
Maximum number of participants 15
Assignment procedure Assignment according to priority