[ 675ARSPBMM14 ] Module Basic Methods of Molecular Biology

Workload Mode of examination Education level Study areas Responsible person Coordinating university
8 ECTS Accumulative module examination B3 - Bachelor's programme 3. year (*)Bioinformatik Tomas Dolezal USB Budweis
Detailed information
Original study plan Bachelor's programme Bioinformatics 2015W
Objectives Objective of the course is to introduce basic molecular biology techniques to students without regarding their orientation either to experimental or so called "green" biology. The molecular techniques which are commonly used by all biology fields will be presented.
Subject 1. Basic orientation in molecular lab - equipment, chemicals and preparation of reagents.
2. Working with sequences - basic rules and terms, databases and working with sequences in computer.
3. Isolation of various types of nucleic acids; gel electrophoresis
4. DNA cloning - various approaches, vectors, restriction endonucleases, cloning design
5. PCR and its applications
6. Genetically modified organisms - safety training.
7. Nucleic acids labelling and detection, hybridization, blotting
8. DNA sequencing, "next generation sequencing"
9. Polymorphism and genetic diversity analysis, homologous genes identification
10. Principles of expression analysis at RNA and protein levels
11. Proteomics, metabolomics, mass spectroscopy
Subordinated subjects, modules and lectures