[ 863BIBCEM112 ] Module Electron Microscopy I

Workload Mode of examination Education level Study areas Responsible person Coordinating university
4 ECTS Accumulative module examination M2 - Master's programme 2. year (*)Biologische Chemie Jana Nebesářová USB Budweis
Detailed information
Pre-requisites For safety reasons: certified 32 ECTS points in chemical laboratory exercises with experimental work carried out by the student.
Original study plan Master's programme Joint Master Programme Biological Chemistry 2018W
Objectives The course objective includes educating students in electron microscopy. To provide students with practical, hands-on experience in electron microscopy.
Subject Individual lectures will focus on:

1. Physical principles of electron microscopy, physical properties of accelerated electrons, resolution, magnification

2. Transmission electron microscope, electromagnetic lenses and their defects, instrument construction

3. Sample preparation for TEM by physical methods, kryomethods, microwaves

4. Sample preparation for TEM by chemical methods, fixation, dehydration, watering

5. Preparation of ultralight cuts, ultramicrotom, knives, contrasting, underlayer foil

6. Scanning electron microscopy, instrument construction

7. Sample preparation for SEM, drying, metal plating

8. Electronmicroscopic cytology

9. Artifacts in TEM and SEM caused by preparation, instrumentation, topography process

10. Digital and photographical recording of microscopic picture

Preparing half-thin cuts, modification of nets and covering by formed membranes, the visualization of plant virus with the method of reversed contrasting, preparing samples for SEM, working with TEM and SEM, viewing prepared samples.

Further information Recommended reading:

MJ Dykstra, LE Reuss: Biological Electron Microscopy – Theory, Techniques and Troubleshooting. 2nd Edition. Kluwer Academic Plenum Publisher. ISBN 0-306-47749-1. 2003

Before each lecture, students will be given a test to show the student has prepared in advance for class.

Corresponding lecture 863ABBCEM112: Modul Electron Microscopy I (4 ECTS)
Subordinated subjects, modules and lectures