[ 863FCTBBITV10 ] VL Biochemical Laboratory Techniques
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| Workload |
Education level |
Study areas |
Responsible person |
Hours per week |
Coordinating university |
| 1,3 ECTS |
M1 - Master's programme 1. year |
(*)Biologische Chemie |
Wolfgang Schöfberger |
1 hpw |
Johannes Kepler University Linz |
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| Detailed information |
| Original study plan |
Master's programme Joint Master Programme Biological Chemistry 2015W |
| Objectives |
Students will learn the modern technologies behind bio-technological production of proteins, nucleic acids and lipids
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| Subject |
Biochemical technology
- Definition and industrial applications of biochemical technology
- Definition I: biopolymers (protein, DNA, carbohydrates. lipids) are subjected to chemical modification or genetic engineering to enhance their functional properties and tailor them to specific applications in industry or healthcare
- Definition II: generation of enzymes that are used as tools for the bio-assisted industrial synthesis of vitamins or oligosaccharides
Proteins
- Peptide synthesis (review)
- Synthesis of semisynthetic proteins (expressed protein ligation)
- Genetic strategies for protein engineering
- Chemical tools for targeted modifications of proteins
- Industrial application: PEGylation of therapeutic proteins
- Industrial application: Generation of engineered lipases for application in detergents
Nucleic Acids
- Solid Phase Oligonucleotide Synthesis (chemical differences to biosynthesis)
- Chemical synthesis of RNA
- Industrial application: Synthesis of modified bases carrying fluorophores and chemical tags for immobilization of relevance in DNA microarrays and sequencing technology
Lipids
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| Criteria for evaluation |
Final examination
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| Methods |
Lectures and discussion sessions
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| Language |
English |
| Changing subject? |
No |
| Corresponding lecture |
863FCTBBITV10: VL Biochemical Technologies (1,3 ECTS)
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| On-site course |
| Maximum number of participants |
- |
| Assignment procedure |
Direct assignment |
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