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VL (*)Cell Line Cultures in Vitro
Versionsauswahl
(*) Unfortunately this information is not available in english.
Workload Education level Study areas Responsible person Hours per week Coordinating university
2 ECTS M2 - Master's programme 2. year Chemistry Jan Kopecký 1 hpw SBU Budweis
Detailed information
Original study plan Master's programme Joint Master Programme Biological Chemistry 2010W
Objectives (*)To familiarize students with the procedural processes of working with animal tissue cultures.
Subject (*)Lecture topics and exercise topics

1. Types of tissue cultures, cell growth parameters

2. Nutritional requirements, culture media

3. Tissue culture laboratory equipment

4. Primary culture of guinea pig kidney

5. Cell lines, passaging and culture conditions

6. Cell cryopreservation

7. Contamination of cell cultures

8. Interactions of animal viruses with cells, detection of virus replication

9. Monoclonal antibody technology (theory)

10. Immunization of laboratory mice, culture of myeloma cells, preparation of feeder cells

11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium

12. Cloning and storage of hybridomas

13. Production of monoclonal antibodies in vivo and in vitro.

Criteria for evaluation (*)Oral Examination
Methods (*)Lecture
Language  English
Changing subject? No
Further information (*)Recommended reading:

J. Davis, ed..: Basic Cell Culture: A Practical Approach, 2nd ed., Oxford University Press, 2002, 416 pp.

On-site course
Maximum number of participants -
Assignment procedure Direct assignment