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VL (*)Electron Microscopy I
Versionsauswahl
(*) Unfortunately this information is not available in english.
Workload Education level Study areas Responsible person Hours per week Coordinating university
3 ECTS M2 - Master's programme 2. year Chemistry Jana Nebesářová 2 hpw SBU Budweis
Detailed information
Original study plan Master's programme Joint Master Programme Biological Chemistry 2010W
Objectives (*)To educate students in electron microscopy
Subject (*)Individual lectures focusing on: 1. Physical principles of electron microscopy, physical properties of accelerated electrons, resolution, magnification

2. Transmission electron microscope, electromagnetic lenses and their defects, instrument construction

3. Sample preparation for TEM by physical methods, kryomethods, microwaves

4. Sample preparation for TEM by chemical methods, fixation, dehydration, watering

5. Preparation of ultralight cuts, ultramicrotom, knives, contrasting, underlayer foil

6. Scanning electron microscopy, instrument construction

7. Sample preparation for SEM, drying, metal plating

8. Electronmicroscopic cytology

9. Artifacts in TEM and SEM caused by preparation, instrumentation, topography process

10. Digital and photographical recording of microscopic picture

Criteria for evaluation (*)Oral examination
Methods (*)Lecture
Language  English
Changing subject? No
Further information (*)Recommended reading:

MJ Dykstra, LE Reuss: Biological Electron Microscopy – Theory, Techniques and Troubleshooting. 2nd Edition. Kluwer Academic Plenum Publisher. ISBN 0-306-47749-1. 2003

On-site course
Maximum number of participants -
Assignment procedure Direct assignment