Inhalt

VL Electron Microscopy I

Versionsauswahl
Workload Ausbildungslevel Studienfachbereich VerantwortlicheR Semesterstunden Anbietende Uni
3 ECTS M2 - Master 2. Jahr Chemie Jana Nebesářová 2 SSt SBU Budweis
Detailinformationen
Quellcurriculum Masterstudium Joint Master Programme "Biological Chemistry" 2010W
Ziele To educate students in electron microscopy
Lehrinhalte Individual lectures focusing on: 1. Physical principles of electron microscopy, physical properties of accelerated electrons, resolution, magnification

2. Transmission electron microscope, electromagnetic lenses and their defects, instrument construction

3. Sample preparation for TEM by physical methods, kryomethods, microwaves

4. Sample preparation for TEM by chemical methods, fixation, dehydration, watering

5. Preparation of ultralight cuts, ultramicrotom, knives, contrasting, underlayer foil

6. Scanning electron microscopy, instrument construction

7. Sample preparation for SEM, drying, metal plating

8. Electronmicroscopic cytology

9. Artifacts in TEM and SEM caused by preparation, instrumentation, topography process

10. Digital and photographical recording of microscopic picture

Beurteilungskriterien Oral examination
Lehrmethoden Lecture
Abhaltungssprache Englisch
Lehrinhalte wechselnd? Nein
Sonstige Informationen Recommended reading:

MJ Dykstra, LE Reuss: Biological Electron Microscopy – Theory, Techniques and Troubleshooting. 2nd Edition. Kluwer Academic Plenum Publisher. ISBN 0-306-47749-1. 2003

Präsenzlehrveranstaltung
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