Specifically, after completing the course, they will be able to:
- recall, understand and explain the experimental methods and applications discussed (k1, k2, k3)
- Analyze, critically examine and solve problems related to the content discussed (k3, k4, k5)
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Students will be introduced to the physical principles of experimental techniques, the required sample preparation, data analysis, and the underlying biophysical models. The lecture content includes:
- Overview of bio-nanostructures: proteins, nucleic acids, biomembranes, viruses, etc.
- Atomic force microscopy (AFM) and high-speed AFM: basic principles, sample preparation strategies, and applications to soluble proteins, membrane proteins, viruses, cells.
- Quartz crystal microbalance (QCM) for real-time characterization of molecular interactions: basic principles, applications and data analysis.
- Single-molecule fluorescence microscopy: principles and applications of optical microscopy, single-molecule and superresolution techniques, fluorescence resonance energy transfer.
- AFM-based single-molecule force spectroscopy and optical tweezers for the characterization of inter- and intramolecular interactions, biophysical models and data evaluation strategies.
- Cryo-electron microscopy (cryo-EM): physical principles and instrumentation, sample preparation, data acquisition, analysis and 3D reconstruction.
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