| Detailed information |
| Pre-requisites |
(*)Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
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| Original study plan |
Doctoral programme Natural Sciences 2025W |
| Learning Outcomes |
Competences |
| Students learn standard single-channel electrophysiology techniques for the characterization of ion channel proteins.
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| Skills |
Knowledge |
| Students acquire practical skills in forming the planar lipid bilayers and using the software for electrophysiological recordings and data analysis. They learn how to incorporate proteins into the planar bilayers
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Folded bilayers; vesicle-bilayer fusion; ion selectivity of channel proteins; single-channel conductivity; voltage gating, analysis of single-channel recordings
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| Criteria for evaluation |
The final grade will be determined by a combination of the students' preparation and execution of the experiment and the quality of the written protocols.
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| Methods |
Experimental techniques in electrophysiology.
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| Language |
English |
| Changing subject? |
No |
| Further information |
The course is part of a comprehensive experimental cycle in membrane transport protein research, covering key stages of investigation. This cycle includes protein production and reconstitution into vesicles (“Overexpression, Purification, and Functional Analysis of Reconstituted Membrane Proteins”), assessment of reconstitution efficiency (“Fluorescence Correlation Spectroscopy”), and functional characterization of the protein (“Single Channel Recordings”). Participation in the other two thematic courses is recommended but not mandatory.
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