Es ist eine neuere Version 2026W dieser LV im Curriculum Doctoral programme Natural Sciences 2026W vorhanden.
(*) Unfortunately this information is not available in english.
Workload
Education level
Study areas
Responsible person
Hours per week
Coordinating university
3 ECTS
R - Doctoral programme
Human Medicine
Susanna Zierler
2 hpw
Johannes Kepler University Linz
Detailed information
Pre-requisites
(*)Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
Original study plan
Doctoral programme Medical Sciences 2025W
Learning Outcomes
Competences
Students acquire theoretical and experimental skills necessary for calcium imaging measurements in cell lines and primary cells.
Skills
Knowledge
Preparing precise intracellular and extracellular imaging solutions
Conducting calcium imaging experiments in cell lines and primary cells
Analyzing and interpreting the obtained results.
Identification of different protocols for distinct scientific questions.
Trouble shooting and optimization of protocols.
Basic understanding of cell physiology and calcium signaling.
Methodologic principles of calcium imaging, including ratiometric calcium dyes and genetically encoded calcium indicators (GECIs).
Knowledge of distinct imaging methods and their applications.
Criteria for evaluation
Attendance of at least 75 %.
Active participation during the course. Teachers will judge on students´ learning behavior and experimental results to give evaluation.
Methods
Introduction and supervision of experimental skills in the electrophysiology and imaging labs
Language
English
Study material
Selected publications provided by the lecturer and autodidactic search of PubMed and other sources.
