| Detailed information |
| Pre-requisites |
(*)Zulassung zum Doktoratsstudium Medical Sciences, Naturwissenschaften bzw. Technischen Wissenschaften
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| Original study plan |
Doctoral programme Medical Sciences 2025W |
| Learning Outcomes |
Competences |
| Students acquire theoretical and experimental skills necessary for calcium imaging measurements in cell lines and primary cells.
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| Skills |
Knowledge |
- Preparing precise intracellular and extracellular imaging solutions
- Conducting calcium imaging experiments in cell lines and primary cells
- Analyzing and interpreting the obtained results.
- Identification of different protocols for distinct scientific questions.
- Trouble shooting and optimization of protocols.
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- Basic understanding of cell physiology and calcium signaling.
- Methodologic principles of calcium imaging, including ratiometric calcium dyes and genetically encoded calcium indicators (GECIs).
- Knowledge of distinct imaging methods and their applications.
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| Criteria for evaluation |
Attendance of at least 75 %.
Active participation during the course. Teachers will judge on students´ learning behavior and experimental results to give evaluation.
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| Methods |
Introduction and supervision of experimental skills in the electrophysiology and imaging labs
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| Language |
English |
| Study material |
Selected publications provided by the lecturer and autodidactic search of PubMed and other sources.
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| Changing subject? |
No |
