Inhalt
[ 405ERSLMSBP25 ] PR Methods of Stem Cell Biology
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| Workload |
Education level |
Study areas |
Responsible person |
Hours per week |
Coordinating university |
| 2,5 ECTS |
R - Doctoral programme |
Human Medicine |
Ahmad Salti |
2 hpw |
Johannes Kepler University Linz |
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| Detailed information |
| Original study plan |
Doctoral programme Medical Sciences 2025W |
| Learning Outcomes |
Competences |
| Students understand induced pluripotent stem cells (iPSCs), their reprogramming, and their applications in regenerative medicine. They can differentiate iPSCs into neural stem cells and identify neuronal morphologies using advanced imaging and molecular techniques. Additionally, they can design experiments, manage time effectively, and analyze data critically.
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| Skills |
Knowledge |
- Perform essential stem cell culture techniques, including thawing, passaging, freezing, and cell counting.
- Characterize cell lines through phase contrast imaging, immunofluorescence (IF), and quantitative real-time PCR (qRT-PCR).
- Differentiate iPSCs into neural stem cells
- Analyze cell morphology using microscopy and imaging tools.
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- Fundamentals of stem cell biology
- Organoid development
- Cell differentiation and characterization
- Molecular techniques (qRT-PCR, immunofluorescence).
- Application of imaging techniques
- Time management
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| Criteria for evaluation |
Attendance of at least 80 %
Presentation and final report about their results during the practical course.
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| Methods |
Lecture and experiential learning through lab experiments.
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| Language |
English |
| Changing subject? |
No |
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| On-site course |
| Maximum number of participants |
8 |
| Assignment procedure |
Assignment according to priority |
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