| Detailed information |
| Pre-requisites |
(*)Zulassung zum Doktoratsstudium Medical Sciences
Masterstudium Biological Chemistry Masterstudium Molecular Biology
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| Original study plan |
Doctoral programme Medical Sciences 2025W |
| Learning Outcomes |
Competences |
| Students have a fundamental understanding of mass spectrometry-based bioanalytical techniques in clinical chemistry, biomedical research, and clinical studies.
Students can use modern preanalytic methods to prepare protein samples for LC-MS/MS analysis. Finally, they can use scientific software to process MS raw data, resulting in qualitative and quantitative information.
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| Skills |
Knowledge |
- Preparing protein samples for bioanalysis.
- Conducting laboratory-based bioanalyses with state-of-the-art LC-MS/MS.
- Using bioinformatics tools to process and interpret mass spectrometry data.
- Presenting and critically discussing peer-reviewed publications.
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- Fundamentals of bioanalytics and clinical chemistry.
- Principles and applications of mass spectrometry in biomedical research.
- Preanalytical workflows for peptide/protein analysis.
- Computational biology and bioinformatics for MS data processing.
- Interpretation of qualitative and quantitative mass spectrometry results.
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| Criteria for evaluation |
At minimum, 80% attendance in each part is required. The journal club is successfully completed after presentation and discussion of peer-reviewed publications from the perspective of biomedical application scientists. Lab work is successfully completed at the end of each workflow and quality assessment of processed samples. Suitable samples will be subjected to LC-MS/MS and subsequently processed by participants. Bioinformatics/computational biology is successfully completed after processing raw LC-MS/MS data to the level of qualitative and quantitative data and its presentation.
Successful participants will be awarded a Training Certificate in Fundamentals in Gold Standard Bioanalytics in Medicine by Mass Spectrometry: from Peptide to Deep Proteome Analyses.
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| Methods |
Hands-on laboratory course accompanied by seminars, journal club and computational lab training.
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| Language |
English |
| Study material |
e.g. Bioanalytik edited by J Kurreck, JW Engels, F Lottspeich
Manuscripts for the journal club will be distributed about two weeks before the beginning of the course.
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| Changing subject? |
No |
| Further information |
Bringing your own protein sample is encouraged not mandatory. Please contact us for a suitable sample buffer. Hands-on opportunity for liquid handling robotics. Demonstration of real-time bioanalytics by ultra high performance liquid chromatography-coupled nanospray ionization fourier transformed tandem mass spectrometry (UHPLC-NSI-FTMS/MS).
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