- Understanding the principles of flow cytometry, fluorophores, spectral overlap and compensation. (k2)
- Plan flow cytometry experiments, including panel design and control selection. (k3, k6)
- Perform surface and intracellular staining of cells. (k3)
- Set up and operate a flow cytometer and troubleshoot common technical issues during data acquisition. (k3, k4)
- Use flow cytometry analysis software (e.g., FlowJo, FCS Express) (k3)
- Analyse, interpret and present data, including histograms, dot plots, and gating hierarchies. (k4, k5)
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- Principles of flow cytometry and fluorophores
- Spectral overlap, compensation, spillover spreading
- Instrument setup and optimization
- Experiment setup and staining protocols
- Data visualization techniques, including histograms, dot plots, and gating strategies
- Applications of flow cytometry in clinical and biomedical research
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