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[ 863STBTSTB24 ] Module Structural Biochemistry
Versionsauswahl
Workload Mode of examination Education level Study areas Responsible person Coordinating university
4 ECTS Accumulative module examination M1 - Master's programme 1. year (*)Biologische Chemie Jan Sterba USB Budweis
Detailed information
Original study plan Master's programme Biological Chemistry 2024W
Objectives The lectures will give students an insight into why it is important to understand the function of macromolecular structures at the molecular level. There have been breakthroughs in modern biochemistry with the development of techniques such as XRD, NMR and EM that allow the structures of macromolecules to be determined. Today, students commonly use structural models of proteins and nucleic acids available in computer databases. These models provide an illustrative but often idealised representation of biological macromolecules. It is therefore crucial that students of modern biochemistry become familiar with structural analysis methods during their studies. By doing so, they will be able to distinguish between what has been measured and what has been calculated and thus better understand the difference between reality and the model.
Subject Content of Lectures: Chemical structure and geometry, conformation and energy (Michal Kutý) Structure of proteins (Ivana Kutá Smatanová, Michal Kutý) Structure of nucleic acids (Tomáš Fessl) Structure of polysaccharides and biological membranes (František Vácha) Computational methods and molecular modelling (Michal Kutý) X-ray crystallography (Pavlína Řezáčová) Nuclear Magnetic Resonance (Vašek Veverka) Electron microscopy (Zdenko Gardian) Mass spectroscopy (Filip Dyčka, Peter Koník) Absorption and fluorescence spectroscopy (Radek Litvín) Optical microscopy and CD (Tomáš Fessl) New trends in structural biochemistry (Ivana Kutá Smatanová)
Further information Content of Practicals: (Barbora Kaščáková, Petra Havlíčková) The knowledge acquired during the lectures will be deepened during the exercise. Solving the structure of a selected protein, visualization using Pymol, using a program for structure prediction (AlphaFold)

Criteria for evaluation The final exam will take the form of oral interview with the final evaluation in the form of awarding the mark

Methods Monologic (reading, lecture, briefing), Dialogic (discussion, interview, brainstorming), Work with text (with textbook, with book), Individual tutoring

Study Material Kensal E. van Holde, W. Curtis Johnson, P. Shing Ho: Principles of physical biochemistry 2nd ed. Pearson Education, Inc. (2006), ISBN 0-13-046427-9

Language English

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