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[ 663BICHBL112 ] Module Biochemistry Laboratory 1
Versionsauswahl
Workload Mode of examination Education level Study areas Responsible person Coordinating university
3 ECTS Accumulative module examination B2 - Bachelor's programme 2. year (*)Biologische Chemie Hana Tykalova USB Budweis
Detailed information
Pre-requisites PR Lab Course in General Chemistry
Original study plan Bachelor's programme Biological Chemistry 2023W
Objectives The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Subject The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
Further information Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification. Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography. DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA. Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes. Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction. SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
Corresponding lecture ANTBIOCBC1U10: PR Biochemisches Praktikum 1 (3 ECTS)
Subordinated subjects, modules and lectures