| Detailed information |
| Pre-requisites |
Molecular Biology and Genetics? (Modul)
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| Original study plan |
Bachelor's programme Biological Chemistry 2023W |
| Objectives |
This course is primarily intended for bachelor students of joint Linz-Ceske Budejovice - Biological chemistry - program. The students will get acquainted with basic laboratory work and molecular biology methods.
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| Subject |
fundamental methods in molecular biology in practice
|
| Further information |
Content of the course
1. Orientation in the molecular biology laboratory
2. Isolation of genomic DNA (gDNA), isolation of RNA
3. Gel electrophoresis
4. Reverse transcription
5. Polymerase chain reaction (PCR)
6. Cloning - ligation of the PCR product with the plasmid
7. Screening of recombinants
8. Isolation of plasmid DNA (pDNA)
9. Sequencing (theoretical)
10. Analysis of the results, guides for "How to make a protocol".
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| Corresponding lecture |
ANTMOBIMMBU10: PR Praktikum zu Methoden der Molekularbiologie (4 ECTS) (as lecture)
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