Inhalt
[ 663BICHBL112 ] Modul (*)Biochemistry Laboratory 1
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| (*) Leider ist diese Information in Deutsch nicht verfügbar. |
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| Workload |
Form der Prüfung |
Ausbildungslevel |
Studienfachbereich |
VerantwortlicheR |
Anbietende Uni |
| 3 ECTS |
Kumulative Modulprüfung |
B2 - Bachelor 2. Jahr |
Biologische Chemie |
Hana Tykalova |
USB Budweis |
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| Detailinformationen |
| Anmeldevoraussetzungen |
(*)PR Lab Course in General Chemistry
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| Quellcurriculum |
Bachelorstudium Biological Chemistry 2023W |
| Ziele |
(*)The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
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| Lehrinhalte |
(*)The laboratory course focuses on the selected topics in analytical and preparative biochemistry while teaching basic techniques needed for work in the biochemistry laboratory.
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| Sonstige Informationen |
(*)Purification of proteins - precipitation with salts and organic solvents, dialysis followed by chromatographic purification.
Chromatographic methods in Biochemistry - separation of proteins using gel chromatography, ionex chromatography, and affinity chromatography.
DNA isolation and analysis - Isolation of plasmid DNA, chromosomal DNA, measurement of DNA concentration, measurement of DNA concentration in microvolumes, agarose gel electrophoresis of DNA.
Methods for protein concentration determination - quantification of proteins using the Lowry method, biuret method, Bradford method, direct determination of protein concentration, direct determination of protein concentration in microvolumes.
Enzyme kinetics - kinetic parameters of protein cleavage by trypsin, kinetic parameters of hydrogen peroxide cleavage by catalase, kinetic parameters of peroxidase reaction.
SDS-PAGE of proteins - electrophoretic separation of proteins in polyacrylamide gels of different concentrations, staining of the separated proteins using the CBB dye.
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| Äquivalenzen |
(*)ANTBIOCBC1U10: PR Biochemisches Praktikum 1 (3 ECTS)
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| Untergeordnete Studienfächer, Module und Lehrveranstaltungen |
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