[ 663MOBIMMB12 ] Module Methods in Molecular Biology

Workload Mode of examination Education level Study areas Responsible person Coordinating university
4 ECTS Accumulative module examination B2 - Bachelor's programme 2. year (*)Biologische Chemie Lenka Chodakova USB Budweis
Detailed information
Pre-requisites Molecular Biology and Genetics? (Modul)
Original study plan Bachelor's programme Biological Chemistry 2023W
Objectives This course is primarily intended for bachelor students of joint Linz-Ceske Budejovice - Biological chemistry - program. The students will get acquainted with basic laboratory work and molecular biology methods.
Subject fundamental methods in molecular biology in practice
Further information Content of the course 1. Orientation in the molecular biology laboratory 2. Isolation of genomic DNA (gDNA), isolation of RNA 3. Gel electrophoresis 4. Reverse transcription 5. Polymerase chain reaction (PCR) 6. Cloning - ligation of the PCR product with the plasmid 7. Screening of recombinants 8. Isolation of plasmid DNA (pDNA) 9. Sequencing (theoretical) 10. Analysis of the results, guides for "How to make a protocol".
Corresponding lecture ANTMOBIMMBU10: PR Praktikum zu Methoden der Molekularbiologie (4 ECTS) (as lecture)
Subordinated subjects, modules and lectures