[ 863STBTCLC12 ] Modul (*)Cell Line Cultures in Vitro

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Workload Form der Prüfung Ausbildungslevel Studienfachbereich VerantwortlicheR Anbietende Uni
3 ECTS Kumulative Modulprüfung M2 - Master 2. Jahr Biologische Chemie Jan Kopecký USB Budweis
Anmeldevoraussetzungen (*)For safety reasons: certified 32 ECTS points in chemical and/or microbiological laboratory exercises with experimental work carried out by the student.
Quellcurriculum Masterstudium Joint Master Programme Biological Chemistry 2015W
Ziele (*)The course aims to familiarize students with the procedural processes of working with animal tissue cultures. Course objectives include acquiring practical, hands-on experience in cell line cultures in vitro.
Lehrinhalte (*)Lecture and exercise topics:

1. Types of tissue cultures, cell growth parameters

2. Nutritional requirements, culture media

3. Tissue culture laboratory equipment

4. Primary culture of guinea pig kidney

5. Cell lines, passaging and culture conditions

6. Cell cryopreservation

7. Contamination of cell cultures

8. Interactions of animal viruses with cells, detection of virus replication

9. Monoclonal antibody technology (theory)

10. Immunization of laboratory mice, culture of myeloma cells, preparation of feeder cells

11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium

12. Cloning and storage of hybridomas

13. Production of monoclonal antibodies in vivo and in vitro.

Application and discussion of recently acquired knowledge from course on Cell Line Cultures in Vitro

Sonstige Informationen (*)Recommended reading:

J. Davis, ed..: Basic Cell Culture: A Practical Approach, 2nd ed., Oxford University Press, 2002, 416 pp.

Äquivalenzen (*)VL Cell Line Cultures in Vitro (2 ECTS) + PR Practical Cell Line Cultures in Vitro (1 ECTS) (as lecture)
Untergeordnete Studienfächer, Module und Lehrveranstaltungen