[ 863STBTCLC12 ] Module Cell Line Cultures in Vitro

Workload Mode of examination Education level Study areas Responsible person Coordinating university
3 ECTS Accumulative module examination M2 - Master's programme 2. year (*)Biologische Chemie Jan Kopeck├Ż USB Budweis
Detailed information
Pre-requisites For safety reasons: certified 32 ECTS points in chemical and/or microbiological laboratory exercises with experimental work carried out by the student.
Original study plan Master's programme Joint Master Programme Biological Chemistry 2015W
Objectives The course aims to familiarize students with the procedural processes of working with animal tissue cultures. Course objectives include acquiring practical, hands-on experience in cell line cultures in vitro.
Subject Lecture and exercise topics:

1. Types of tissue cultures, cell growth parameters

2. Nutritional requirements, culture media

3. Tissue culture laboratory equipment

4. Primary culture of guinea pig kidney

5. Cell lines, passaging and culture conditions

6. Cell cryopreservation

7. Contamination of cell cultures

8. Interactions of animal viruses with cells, detection of virus replication

9. Monoclonal antibody technology (theory)

10. Immunization of laboratory mice, culture of myeloma cells, preparation of feeder cells

11. Fusion of splenic lymphocytes with myeloma cells and cultivation in the selection medium

12. Cloning and storage of hybridomas

13. Production of monoclonal antibodies in vivo and in vitro.

Application and discussion of recently acquired knowledge from course on Cell Line Cultures in Vitro

Further information Recommended reading:

J. Davis, ed..: Basic Cell Culture: A Practical Approach, 2nd ed., Oxford University Press, 2002, 416 pp.

Corresponding lecture VL Cell Line Cultures in Vitro (2 ECTS) + PR Practical Cell Line Cultures in Vitro (1 ECTS) (as lecture)
Subordinated subjects, modules and lectures