[ 863BIBCEMP21 ] Modul (*)Experimental Methods in Protein Biochemistry

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Workload Form der Prüfung Ausbildungslevel Studienfachbereich VerantwortlicheR Anbietende Uni
4 ECTS Kumulative Modulprüfung M2 - Master 2. Jahr Biologische Chemie USB Budweis
Quellcurriculum Masterstudium Biological Chemistry 2021W
Ziele (*)The course focuses on the chemistry of peptides and proteins as well as physical and chemical methods applied to the studies of protein structure, protein structures in relation to their function, and the structure of proteins.

Students will acquire practical hands-on experience in the field of protein chemistry.

Lehrinhalte (*)The course reviews the chemistry of peptides and proteins: Polymer of amino acid, the backbone of a polymer; Three-dimensional structure: Secondary structure, Fibrous proteins, Globular proteins, Protein stability, Quaternary structure; Protein folding and dynamics: Protein folding, theory and experiment, protein denaturation, folding pathway and prediction of protein structure; Protein dynamics; atomic fluctuations, collective motions and triggered, conformational changes; Experimental methods applied to study protein structures: Transport in electric field, Scattering by larger particles (X-rays diffraction by fibrous proteins, NMR, Circular dichroism, Laser raman, Neutron diffraction; Theoretical methods applied to study protein structures: homolgy modeling, molecular dynamics, ligand/docking, coarse graining; Allosteric regulation; Adair equation, Symmetry model, Sequential model and cooperativity.

Exercising and discussing recently learned material from the course in protein chemistry.

Sonstige Informationen (*)Learning Goals. Students will:

• acquire detailed insight into the structure of proteins with respect to protein chemical and biophysical experiments

• acquire insight into the use of prediction methods and molecular modeling techniques

• acquire knowledge and insight into protein folding in vitro and comparison to in vivo protein folding

• acquire knowledge and insight into the general principles of protein purification (based on size, charge, hydrophobicity or on biospecific properties)

• acquire knowledge of possible chemical modification reactions

Äquivalenzen (*)863BIBCPRC12: Modul Protein Chemistry (4 ECTS)
Untergeordnete Studienfächer, Module und Lehrveranstaltungen