[ 863BIBCEM112 ] Modul (*)Electron Microscopy I

(*) Leider ist diese Information in Deutsch nicht verfügbar.
Workload Form der Prüfung Ausbildungslevel Studienfachbereich VerantwortlicheR Anbietende Uni
4 ECTS Kumulative Modulprüfung M2 - Master 2. Jahr Biologische Chemie Jana Nebesářová USB Budweis
Anmeldevoraussetzungen (*)For safety reasons: certified 32 ECTS points in chemical laboratory exercises with experimental work carried out by the student.
Quellcurriculum Masterstudium Joint Master Programme Biological Chemistry 2018W
Ziele (*)The course objective includes educating students in electron microscopy. To provide students with practical, hands-on experience in electron microscopy.
Lehrinhalte (*)Individual lectures will focus on:

1. Physical principles of electron microscopy, physical properties of accelerated electrons, resolution, magnification

2. Transmission electron microscope, electromagnetic lenses and their defects, instrument construction

3. Sample preparation for TEM by physical methods, kryomethods, microwaves

4. Sample preparation for TEM by chemical methods, fixation, dehydration, watering

5. Preparation of ultralight cuts, ultramicrotom, knives, contrasting, underlayer foil

6. Scanning electron microscopy, instrument construction

7. Sample preparation for SEM, drying, metal plating

8. Electronmicroscopic cytology

9. Artifacts in TEM and SEM caused by preparation, instrumentation, topography process

10. Digital and photographical recording of microscopic picture

Preparing half-thin cuts, modification of nets and covering by formed membranes, the visualization of plant virus with the method of reversed contrasting, preparing samples for SEM, working with TEM and SEM, viewing prepared samples.

Sonstige Informationen (*)Recommended reading:

MJ Dykstra, LE Reuss: Biological Electron Microscopy – Theory, Techniques and Troubleshooting. 2nd Edition. Kluwer Academic Plenum Publisher. ISBN 0-306-47749-1. 2003

Before each lecture, students will be given a test to show the student has prepared in advance for class.

Äquivalenzen (*)863ABBCEM112: Modul Electron Microscopy I (4 ECTS)
Untergeordnete Studienfächer, Module und Lehrveranstaltungen