[ 863CTBCBLTV19 ] VL (*)Biochemical Laboratory Techniques

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Workload Ausbildungslevel Studienfachbereich VerantwortlicheR Semesterstunden Anbietende Uni
1,5 ECTS M1 - Master 1. Jahr Biologische Chemie Wolfgang Schöfberger 1 SSt Johannes Kepler Universität Linz
Quellcurriculum Masterstudium Biological Chemistry 2019W
Ziele (*)Students will learn the modern technologies behind bio-technological production of proteins, nucleic acids and lipids
Lehrinhalte (*)Biochemical technology

  • Definition and industrial applications of biochemical technology
  • Definition I: biopolymers (protein, DNA, carbohydrates, lipids) are subjected to chemical modification or genetic engineering to enhance their functional properties and tailor them to specific applications in industry or healthcare
  • Definition II: generation of enzymes that are used as tools for the bio-assisted industrial synthesis of vitamins or oligosaccharides


  • Peptide synthesis (review)
  • Synthesis of semisynthetic proteins (expressed protein ligation)
  • Genetic strategies for protein engineering
  • Chemical tools for targeted modifications of proteins
  • Industrial application: PEGylation of therapeutic proteins
  • Industrial application: Generation of engineered lipases for application in detergents

Nucleic Acids

  • Solid Phase Oligonucleotide Synthesis (chemical differences to biosynthesis)
  • Chemical synthesis of RNA
  • Industrial application: Synthesis of modified bases carrying fluorophores and chemical tags for immobilization of relevance in DNA microarrays and sequencing technology


  • Application: Biofuels
Beurteilungskriterien (*)Final examination
Lehrmethoden (*)Lectures and discussion sessions
Abhaltungssprache Englisch
Lehrinhalte wechselnd? Nein
Äquivalenzen (*)863FCTBBITV10: VL Biochemical Laboratory Techniques (1.3 ECTS)
Teilungsziffer -
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