Inhalt

[ 663MAPHBPHP18 ] PR Biophysics Laboratory for Biological Chemistry

Versionsauswahl
Workload Education level Study areas Responsible person Hours per week Coordinating university
3 ECTS B3 - Bachelor's programme 3. year Physics Andreas Horner 2 hpw Johannes Kepler University Linz
Detailed information
Original study plan Bachelor's programme Biological Chemistry 2018W
Objectives The biophysical laboratory aims at conveying students with basic knowledge in Biophysics.
Subject Students have to conduct four out of five offered experiments with a scope as diverse as:
(i) Electrical characterization of a free standing planar lipid bilayers with a two electrode setup and measuring the single channel conductivity and life time of reconstituted gramicidin A.
(ii) Estimating the critical micelle concentration of a detergent and its complexing stoichiometry with β-cyclodextrin with the help of contact angle measurements on water droplets.
(iii) Measuring the binding affinity of a fluorescent probe to lipid vesicles and calculating the respective surface charge density and surface potential from it.
(iv) Analyzing the membrane fusion of lipid vesicles via turbimetry, fluorescence resonance energy transfer and fluorescent self-quenching to be able to differentiate between aggregation, semi-fusion and fusion.
(v) Estimating weak acid permeation across planar lipid bilayers from the transmembrane potential generated in the presence of a proton carrier.
Criteria for evaluation Written and oral examination
Language English
Changing subject? No
Further information
  • Saparov et al. A New Model of Weak Acid Permeation through Membranes Revisited: Does Overton Still Rule?. Biophysical Journal 90, 11, 2006, 86-88
  • Gibrat et al. A procedure for estimating the surface potential of charged or neutral membranes with 8-anilino-1-naphthalenesulphonate probe. Adequacy of the Gouy-Chapman model. BBA 736, 2, 1983, 196-202
  • Kaufmann et al. A Novel Method for Detergent Concentration Determination. Biophysical Journal. 90, 1, 2006, 310-317
  • Wolley et al. Model ion channels: Gramicidin and alamethicin. JMB 129, 2, 1992, 109-136
  • Arnold. Cation-Induced Vesicle Fusion modulated by polymers and proteins. Handbook of Biological Physics 19, 1, 1995
Corresponding lecture BCBPMPRBPHY: PR Biophysics Laboratory (3 ECTS)
On-site course
Maximum number of participants 10
Assignment procedure Direct assignment