Inhalt
[ 675ARSPMMB13 ] Module Methods in Molecular Biology
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Mode of examination |
Education level |
Study areas |
Responsible person |
Coordinating university |
| 8 ECTS |
Accumulative module examination |
B1 - Bachelor's programme 1. year |
(*)Bioinformatik |
Tomas Dolezal |
USB Budweis |
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| Detailed information |
| Original study plan |
Bachelor's programme Bioinformatics 2015W |
| Objectives |
This course is for students who need to expand their knowledge from theoretical classes with practical experience at the bench. Up to 20 offered methods cover both basic and advanced approaches, used by researchers at institutes of the Academy of Sciences. Some of these methods are unique in the Czech Republic as they utilize model organisms Drosophila melanogaster and C. elegans.
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Theoretical lectures will explain principles of the methods and their application.
The following topics will be covered:
- Recombinant DNA, cloning vectors, gene libraries.
- Purification and labeling of nucleic acids, hybridization.
- DNA modifying enzymes, cloning strategies, DNA sequencing.
- PCR applications.
- Analyses of mRNA, S1 mapping and primer extension.
- Analyses of gene expression at the mRNA level: in situ hybridization, RT-PCR, quantitative RT-PCR, microarray technology.
- Extraction and separation of proteins.
- Design and preparation of recombinant proteins in E. coli, fusion and tagged proteins.
- Antibodies, immunodetection methods.
- Analyses of gene expression at the protein level: western blotting, immunocytochemistry.
- Protein-protein interaction analyses: GST pull-down assay, immunoprecipitation, yeast two-hybrid system.
- Protein-DNA interaction analyses: EMSA, DNAse protection assay.
- Analyses of transcriptional regulation in cell transfection systems.
- Analyses of gene expression and function in transgenic eukaryotic models (Drosophila and C. elegans).
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