Inhalt
[ 675ARSPEPG13 ] Module Epigenetics
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| Workload |
Mode of examination |
Education level |
Study areas |
Responsible person |
Coordinating university |
| 3 ECTS |
Accumulative module examination |
B1 - Bachelor's programme 1. year |
(*)Bioinformatik |
Petr Svoboda |
USB Budweis |
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| Detailed information |
| Original study plan |
Bachelor's programme Bioinformatics 2015W |
| Objectives |
To explain principles and means of epigenetic recording and transfer of information.
To make students aware of epigenetic mechanisms in eucaryotic models and during cell development
To motivate students to solve problems
To give students a chance to practice code of academic integrity.
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| Subject |
Introduction - overview of the course, basic concepts of epigenetic marks, diversity of epigenetic mechanisms and effects
Histone modification I - concept of chromatin structure. Heterochromatin and euchromatin. Core histones, linker histones, replacement histones, protamines. Methods for studying chromatin.
Histone modification II - histone modifications, polycomb proteins, acetylation, fosforylation and histone methylations, effects on gene expression.
DNA methylation I - molecular basis of DNA methylation. CpG and non-CpG methylation. Adenosin methylation. Metods for studying DNA methylation. Bisulfite sequencing.
DNA methylation II - effects of DNA methylation on gene expression, Methyl-binding proteins and mechanisms of inhibition of gene expression, distribution of DNA methylation within genes and mammalian genomes.
Imprinting - concept of imprinting, mammalian imprinting. Molecular mechanisms of imprinting. Role of imprinting, Battle of the sexes.
X-inactivation - principles and different strategies for dosage compensation. Control of X-inactivation in mammals.
Epigenetic reprogramming in mammalian life-cycle - integration of epigenetic modification in the mammalian life cycle. Reprogramming of gene expression during development, artificial reprogramming - the traditional view.
Epigenetic mechanisms found in other model systems (plants, yeasts, invertebrates ?) - selected epigenetic mechanisms controlling genome integrity and gene expression
RNA silencing I - molecular machines for RNA silencing - "historical" introduction to RNA silencing. Post-transcriptional effects. Roles and effects of dsRNA. Proteins and complexes in RNA silencing.
RNA silencing II - RNAi technology - experimental and therapeutic use. Design of RNAi experiments
RNA silencing III - roles of RNA silencing pathways - miRNA pathway, chromatin connection.
Integrated view of epigenetic regulation of gene expression - establishment of pluripotency in ES cells and embryos.
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